Investigation of Amikacin, Moxifloxacin and Kanamycin Susceptibility in Mycobacterium tuberculosis Complex Clinical Isolates Susceptible and Multidrug resistant to First-Line Antituberculosis Drugs with Resazurin Microtiter Plate Method
نویسندگان
چکیده
Tüberküloz (TB) dünya çapında en yaygın ölüm nedenlerinden biridir. Çok ilaca dirençli tüberküloz (ÇİD-TB) olguları düşük tedavi başarısı, kötü prognoz ve yüksek maliyetleri ile önemli bir sorundur. Çalışmamızda birinci seçenek anti-TB ilaçlara duyarlı Mycobacterium tuberculosis kompleks (MTC) izolatlarının ÇİD-TB amikasin (AK), moksifloksasin (MOKS) kanamisin (KAN) duyarlılıklarını resazurin mikrotitre plak yöntemi araştırılması amaçlanmıştır. Mikobakteriyoloji laboratuvarı kültür koleksiyonundan 19 izolat izoniazid rifampisine 12 olmak üzere toplam 31 adet MTC izolatı çalışmaya dahil edilmiştir. Duyarlı izolatlarda her üç da direnç saptanmamıştır. izolatlarından üçü (3/12) AK’a, ikisi (2/12) MOKS’a dördü (4/12) KAN’a bulunmuştur. tanesinin olduğu saptanmıştır. izolatları arasında ilaç direnci (YİD-TB) %8 (1/12) olarak tespit gelişiminden sonra YİD-TB görülmesi TB başarısında sorundur tedavide etkin yol izleyebilmek için profillerini belirlemek gereklidir. izolatlarında saptanan AK, MOKS KAN direncinin hem tedavisinin daha sıkı takip edilmesine de yapılacak olan çalışmalara katkı sağlayacağı kanaatindeyiz.
منابع مشابه
Differential protein expression in Mycobacterium tuberculosis susceptible and multidrug resistant isolates
Introduction: Infections by multidrug resistant Mycobacterium tuberculosis (MDR-TB) is a major public health challenge. Secretion of proteins by M. tuberculosis plays an important role in the pathogenesis of the bacterium. We compared the protein profiles of susceptible M. tuberculosis and MDR-TB isolates using proteomic analyses, namely two dimensional gel electrophoresis (2DE) and mass spectr...
متن کاملResazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method.
The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good...
متن کاملComparison of resazurin microtiter assay performance and BACTEC MGIT 960 in the susceptibility testing of Brazilian clinical isolates of Mycobacterium tuberculosis to four first-line drugs
We assessed the performance of REMA in comparison with BACTEC MGIT 960 in the susceptibility testing of 80 Mycobacterium tuberculosis clinical isolates from Clemente Ferreira Institute against four drugs. REMA proved to be a rapid and accurate method, providing excellent correlation with BACTEC MGIT 960, with the exception of results for the ethambutol drug.
متن کاملCo-occurrence of amikacin-resistant and -susceptible Mycobacterium tuberculosis isolates in clinical samples from Beijing, China.
OBJECTIVES This study examined the phenomenon of heteroresistance in Mycobacterium tuberculosis clinical isolates obtained from retreated patients in Beijing, China between 2006 and 2011. METHODS The iPLEX Gold assay platform was used to determine the prevalence of heteroresistance to injectable second-line drugs (amikacin, kanamycin and capreomycin) in resistant isolates. RESULTS Heterores...
متن کاملResazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.
A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ANKEM dergisi
سال: 2022
ISSN: ['1301-3114', '2667-7652']
DOI: https://doi.org/10.54962/ankemderg.1216208